Poly(Lactide-co-glycolide)- poly(ethylene glycol) with dibenzocycolctyne (PLGA-PEG-DBCO) is a linear heterobifunctional PEGylation reagent with a PLGA and an DBCO for Cu-free chemistry. PLGA-PEG-DBCO can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. The reaction of azides with strained alkynes, such as cyclooctynes, readily forms a triazole product without the need for a toxic catalystPEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.
Poly(lactide-co-glycolide)-b-poly(ethylene glycol) ) is one the most commonly used biodegradable amphiphilic block copolymers (ABCs) for drug delivery applications. PEG is the hydrophilic part and PLGA is the hydrophobic part. Amphiphilic block copolymers (AmBC) consist two chemically different homopolymer blocks. One of the block is hydrophilic and the other one is hydrophobic. These macromolecules have the properties to self-assemble into micelles when dissolved in an aqueous media.